Search results for " phylogenetic analyses"

showing 2 items of 2 documents

Rare AU-1-like G3P[9] human rotaviruses with a Kun-like NSP4 gene in children with diarrhea in Italy

2007

ABSTRACT Three G3P[9] rotaviruses, detected in children hospitalized with gastroenteritis in Palermo, Italy, were found to be genetically related to strains of either human or feline origin in the VP7, VP4, and VP6 genes. In contrast, in the NSP4 gene the viruses resembled G2P[4] human strains, suggesting a reassortment between AU-1-like and Kun-like strains.

Microbiology (medical)DiarrheaRotavirusSettore MED/07 - Microbiologia E Microbiologia ClinicaSettore MED/17 - Malattie InfettivevirusesReassortmentMolecular Sequence DataReoviridaeSequence HomologyViral Nonstructural Proteinsmedicine.disease_causeVirusRotavirus InfectionsRotavirus Phylogenetic analysesfluids and secretionsPhylogeneticsRotavirusVirologyGenotypemedicineHumansChildGenePhylogenyViral Structural Proteinsbiologyvirus diseasesSequence Analysis DNAbiology.organism_classificationVirologyDiarrheaItalymedicine.symptom
researchProduct

Epidemiological Survey of Grapevine Leafroll-Associated Virus 1 and 3 in Sicily (Italy): Genetic Structure and Molecular Variability

2022

Background: the most widely distributed and virulent Grapevine leafroll-associated viruses (GLRaV) that affect grapevine are GLRaV-1 and GLRaV-3, transmitted semi-persistently by different mealybugs and soft scales, mainly causing downward rolling of the leaf margins and interveinal reddening. Methods: the main objectives of this study were to investigate the genetic structure and molecular diversity of GLRaV-1 and GLRaV-3 in 617 samples from 11 autochthonous Sicilian grapevine cultivars, ascertaining their presence and spread. The detection was implemented by serological and molecular analyses and subsequently phylogenetic analyses on selected Sicilian isolates were conducted. Results: in …

grapevine disease; GLRaV-1; GLRaV-3; <i>Closteroviridae</i>; <i>Ampelovirus</i>; DAS-ELISA; RT-PCR; phylogenetic analysesPlant ScienceAgronomy and Crop Sciencegrapevine disease GLRaV-1 GLRaV-3 Closteroviridae Ampelovirus DAS-ELISA RT-PCR phylogenetic analysesFood ScienceAgriculture; Volume 12; Issue 5; Pages: 647
researchProduct